The isolation and regeneration of potato (Solanum tuberosum L) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid . The Isolation of regeneratiom of potato (Solanum tuberosum L.) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid. One of new technologies in order to create hybrid plant from different species is protoplast fusion. This technique involves protoplast isolation, purification.

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Callus wich has been two times resubcultured was suspended in the digestive medium without driselase, CaCl2, and MES and incubated in lowlight intensity by constant shaking at rpm in reciprocal water shaker at 30 celcius centigrade. Fusion was induced with PEG solution according to Uchimia and fused protoplasts were collected by centrifugation of g. Protoplast were cultured on the medium of Ikeda and Uchimia.

Get permanent URL for this record. Cell wall generation was observed. Standard Search Advanced Search. Reference Number Reference Number.

Isolation of protoplast from soybean, cowpea, and tobacco and..|INIS

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The first factor was concentrations of sucrose which consists of three levels namely 0. The results showed that there was effect of treatment for all variables observed.

They were incibated in full darkness at 27 Celcius centigrade by constant shaking at 50 rpm orbitor shaker. Leaf protoplast were releasaed in h, soybean and tobacco callus protoplast in h, and cowpeas callus protoplast in h of incubation. Indonesian, English Full-text formats available: Protoplast were collected by centrifugation of g and a thin layer of the suspension was irradiated with ultraviolet light.

Protoplss General Country of publisher: The second factor was incubation time which consists of iso,asi levels namely 2 hours, 4 hours, 6 hours and 8 hours.

Isolasi Protoplas Tanaman Kacang Panjang Secara Enzimatis

For soybean leaf, the medium was enriched with driselase and pectolyase Y Funding for sustainable Open Access incl. Please provide a name for this query: Isolation of protoplast from soybean, cowpea, and tobacco and their fusion.

Journal Homepage Journal Title: The obyectives of the research was to investigate the effect of combination treatment of sucrose concentrations and incubation time on protoplast isolation of the leaves mesophyll orchid of Paraphalaenopsis layockii. Publication Year Publication Year. Data were analyzed using Kruskal-Wallis test followed by Mann-Whitney test. Close Go to Workspace. Results 1 – 1 of 1. Looks like Javascript is disabled on your browser.


Isolasi dan fusi protoplas dari kedelai, buncis, dan tembakau. Regeneration of cell wall observed progessively in mother protoplast from tobacco, cowpea A27, E1, and H6 and fused protoplast of soybean with tobacco, tobacco with cowpea C4, E1, and H6soybean with cowpea C4 and between cowpea C4 and cowpea E1.

Journal on Agriculture Science. The highest protoplast density and number of viable protoplasts achieved in the combined treatment of 0. The factorial experiment consisted of two factors that was arranged by completely randomized design CRD with ten replications. On both medium isllasi protoplast, irradiated protoplasts and their fused do not regenerate cell wall and all cultured died out within four weeks isolazi.